Anti-Gli3 antibody (ab123495)
Key features and details
- Rabbit polyclonal to Gli3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Gli3 antibody
See all Gli3 primary antibodies -
Description
Rabbit polyclonal to Gli3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 875-925 of Human Gli3 using the numbering given in entry NP_000159.3.
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Positive control
- Whole cell lysate from 293T and HeLa
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Gli3 antibody (ab123495)All lanes : Anti-Gli3 antibody (ab123495) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T at 50 µg
Lane 2 : Whole cell lysate from 293T at 15 µg
Lane 3 : Whole cell lysate from Jurkat at 50 µg
Lane 4 : Whole cell lysate from HeLa at 50 µg
Developed using the ECL technique.
Predicted band size: 170 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-Gli3 antibody (ab123495)Immunoprecipitation from 293T whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123495 at 6 µg/mg lysate for IP
Subsequent Western blot detection used ab123495 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
