Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)
![Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)](https://www.abcam.com/ps/products/180/ab180175/Images/ab180175-208838-anti-cd31-phospho-y713-antibody-epr80792-c-terminal-western-blot.jpg "Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8079(2)] to CD31 (phospho Y713) - C-terminal
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal
See all CD31 primary antibodies -
Description
Rabbit monoclonal [EPR8079(2)] to CD31 (phospho Y713) - C-terminal -
Host species
Rabbit -
Specificity
ab180175 recognizes only the Y713-phosphorylated form of human CD31.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human CD31 aa 700 to the C-terminus (C terminal) (phospho Y713) (Cysteine residue). The exact sequence is proprietary.
Database link: P16284 -
Positive control
- WB: Pervanadate-treated Jurkat cell lysate. Dot Blot: CD31 (pY713) peptide.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8079(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)All lanes : Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175) at 1/1000 dilution
Lane 1 : Non-treated Jurkat cell lysate
Lane 2 : Pervanadate-treated Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
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![Dot Blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)](https://www.abcam.com/ps/products/180/ab180175/Images/ab180175-255454-anti-cd31-phospho-y713-antibody-epr80792-c-terminal-dot-blot.jpg)
Dot Blot - Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)Dot blot analysis of CD31 (pY713) peptide (Lane 1) and CD31 non-phospho peptide (Lane 2) labelling CD31 (phospho Y713) with ab180175 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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![Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)](https://www.abcam.com/ps/products/180/ab180175/Images/ab180175-4-benefits-of-recombinant-antibodies.png)
Anti-CD31 (phospho Y713) antibody [EPR8079(2)] - C-terminal (ab180175)
