Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD36 antibody [EPR22509-40] (ab252922)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22509-40] to CD36
Suitable for: WB, IHC-P, Flow Cyt
Reacts with: Rat, Human

Product name

Anti-CD36 antibody [EPR22509-40]
See all CD36 primary antibodies
Description

Rabbit monoclonal [EPR22509-40] to CD36
Host species

Rabbit
Specificity

CD36 expression is low in the normal brain (PMID: 27646002).

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Rat Human
WB	Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U937 whole cell lysate; rat spleen and fat tissue lysates; human lung and placenta tissue lysates. IHC-P: Human spleen and hepatocellular carcinoma tissue; rat stomach tissue. Flow cyt: THP-1 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22509-40
Isotype

IgG
Research areas

Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

Lane 1 : Rat spleen lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 70-110 kDa
why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking buffer: 5% NFDM/TBST

Dilution buffer: 5% NFDM /TBST

CD36 expression is low in the normal brain (PMID:27646002)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunohistochemical analysis of [paraffin-embedded human spleen tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human spleen (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG Fc (Alexa Fluo^r® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1000 cells

Lane 1 : Human placenta tissue lysate
Lane 2 : Human lung tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 53 kDa

Exposure time: 3 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247).
Western blot - Anti-CD36 antibody [EPR22509-40] (ab252922)

All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : Rat spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 53 kDa
Observed band size: 70-110 kDa why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

Negative control: Ramos, Jurkat (PMID: 27956597,10744776)

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247, 8631821).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat stomach. (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] (ab252922)

Immunohistochemical analysis of paraffin-embedded human hepatocellular tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human hepatocellular carcinoma (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cell line labelling CD36 with ab252922 at 1/50 dilution (red) compared with Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150097) at 1/5000 dilution was used as the secondary antibody.

Negative control: Ramos (PMID: 10744776).Gated on viable cells.
Flow Cytometry - Anti-CD36 antibody [EPR22509-40] (ab252922)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG Fc (Alexa Fluo^r® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Negative control: Ramos (PMID: 10744776).
Anti-CD36 antibody [EPR22509-40] (ab252922)

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