Mouse PD-1H ELISA Kit (ab231933)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 44.3 pg/ml
- Range: 390.63 pg/ml - 25000 pg/ml
- Sample type: Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse PD-1H ELISA Kit
See all VISTA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 9 2.9% Inter-assay Sample n Mean SD CV% Serum 3 2.5% -
Sample type
Serum, Tissue Extracts, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
44.3 pg/ml -
Range
390.63 pg/ml - 25000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 120 113% - 123% Tissue Extracts 111 103% - 118% Cell Lysate 106 102% - 110% Hep Plasma 109 105% - 123% EDTA Plasma 117 108% - 123% Cit plasma 107 103% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse PD-1H ELISA Kit (ab231933) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-1H protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, and cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse PD-1H with 44.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse PD-1H Capture Antibody 1 x 600µl 10X Mouse PD-1H Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse PD-1H Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- B7 H5
- B7H5
- C10orf54
see all -
Database links
- Entrez Gene: 74048 Mouse
- SwissProt: Q9D659 Mouse
- Unigene: 273584 Mouse
Images
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Other - Mouse PD-1H ELISA Kit (ab231933)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse PD-1H standard curve in 1X Cell Extraction Buffer PTRThe PD-1H standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PD-1H in mouse serum and plasma samplesThe concentrations of PD-1H were measured in duplicates, interpolated from the PD-1H standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 100%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-1H concentration in the neat samples was determined to be 19.7 ng/mL in serum, 17.8 ng/mL in plasma (citrate), 15.3 ng/mL in plasma (EDTA), and 31.8 ng/mL in plasma (heparin).
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Interpolated concentrations of native PD-1H in mouse spleen extract, lung extract, placenta extract, and RAW 264.7 cell extractThe concentrations of PD-1H were measured in duplicate and interpolated from the PD-1H standard curve and corrected for sample dilution. Undiluted samples are 250 µg/mL for all extracts. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Mouse PD-1H ELISA Kit (ab231933)To learn more about the advantages of recombinant antibodies see here.
