Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22509-40] to CD36 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-CD36 antibody [EPR22509-40] - BSA and Azide free
    See all CD36 primary antibodies

  • Description

    Rabbit monoclonal [EPR22509-40] to CD36 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    CD36 expression is low in the normal brain (PMID: 27646002).

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U937 whole cell lysate; rat spleen and fat tissue lysates; human lung and placenta tissue lysates. IHC-P: Human spleen and hepatocellular carcinoma tissue; rat stomach tissue. Flow cyt: THP-1 cells.

  • General notes

    Ab255331 is the carrier-free version of ab252922. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255331 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
    Western blot - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
    All lanes : Anti-CD36 antibody [EPR22509-40] (ab252922) at 1/1000 dilution

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 70-110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking and dilution buffer: 5% NFDM/TBST

    CD36 expression is low in the normal brain (PMID:27646002).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252922).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat stomach. (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252922).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human hepatocellular carcinoma (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252922).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD36 with ab252922 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human spleen (PMID:30047927,22777768) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252922).

  • Flow Cytometry - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
    Flow Cytometry - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)

    Flow cytometric analysis of  THP-1 (human monocytic leukemia cell line) cells labelingCD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252922).

  • Flow Cytometry - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
    Flow Cytometry - Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)

    Flow cytometric analysis of  Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling CD36 with ab252922 at 1/50 dilution (red) compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Gated on viable cells.

    Negative control: Ramos (PMID: 10744776).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252922).

     

  • Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)
    Anti-CD36 antibody [EPR22509-40] - BSA and Azide free (ab255331)

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