Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6573] to CD36 - Low endotoxin, Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free
    See all CD36 primary antibodies

  • Description

    Rabbit monoclonal [EPR6573] to CD36 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 57% homology with SCARB1. Cross-reactivity with this protein has not been confirmed experimentally. Expression levels of the target protein vary with sample type and some optimisation may be require

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab190596)

  • Positive control

    • WB: 3T3-L1 and NIH 3T3 cell lysates. IP: 3T3-L1 cell lysate. IHC-P: FFPE Ms small intestine tissue sections

  • General notes

    ab221605 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

    Lane 1 : 3T3-L1 cell lysate
    Lane 2 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 53 kDa



    The lysate in this image is prepared by 1%SDS Hot Lysate buffer. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)

    Ab133625 staining CD36 in paraffin embedded Human Hepatocellular cancer tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining on endothelial cells in human hepatocellular cancer.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    All lanes :

    Lanes 1-2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
    Lane 3 : Human adipose lysates
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 5 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates
    Lane 6 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 7 : Mouse liver lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?


    Exposure time: 50 seconds


    The expression level of CD36 varies in different samples, and it could be upregulated by treatments such as PMA and Porphyromonas gingivalis (PMID: 8576181 and 27234131).

    RAW 264.7 and mouse liver are reported to be positive for CD36 by PMID: 26187465 and 26186589, but this antibody failed to detect clear signal in normal conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)

    Ab133625 staining CD36 in paraffin embedded Human cardiac muscle tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining mainly on endothelial cells in human cardiac muscle.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1000 cells

    Lane 1 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates prepared in RIPA lysis method
    Lane 2 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution + HEK293 (human embryonic kidney epithelial cell) transfected with His-tagged human CD36 (30aa-439aa) expression vector, whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 53 kDa
    Observed band size: 74 kDa why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

    Lane 1 : Human Heart Tissue Lysate
    Lane 2 : Human Adipose Tissue Lysate
    Lanes 3-4 : Mouse Adipose Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 53 kDa
    Observed band size: 88 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133625 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution (purified) + NIH/3T3 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 78-88 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    The lysate in this image is prepared by 1%SDS Hot lysis method.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Western blot - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution + NIH/3T3 at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 78-88 kDa why is the actual band size different from the predicted?



    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    The lysate in this image is prepared by 1%SDS Hot Lysate buffer. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Immunoprecipitation - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Immunoprecipitation - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)

    ab133625 (unpurified) at 1/5 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Immunoprecipitation - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Immunoprecipitation - Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)

    ab133625 (purified) at 1/50 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133625).

  • Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)
    Anti-CD36 antibody [EPR6573] - Low endotoxin, Azide free (ab221605)

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