Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸ 10 µl 140 716 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6573] to CD36
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Human

Product name

Anti-CD36 antibody [EPR6573]
See all CD36 primary antibodies
Description

Rabbit monoclonal [EPR6573] to CD36
Host species

Rabbit
Specificity

The immunogen used for this product shares 57% homology with SCARB1. Cross-reactivity with this protein has not been confirmed experimentally. Expression levels of the target protein vary with sample type and some optimisation may be required.

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD36 aa 350-450. The exact sequence is proprietary.
Database link: P16671
(Peptide available as ab190596)
Positive control
- WB: HepG2, 3T3-L1 and NIH 3T3 cell lysates; human adipose tissue and platelet lysates. IP: 3T3-L1 cell lysate. IHC-P: FFPE Mouse small intestine tissue; human cardiac muscle and hepatocellular cancer tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR6573
Isotype

IgG
Research areas

Western blot - Anti-CD36 antibody [EPR6573] (ab133625)

All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates with 5% NFDM/TBST
Lane 2 : THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml PMA (Phorbol-12-myristate-13-acetate) for 72 hours whole cell lysates with 5% NFDM/TBST
Lane 3 : Human adipose lysates with 5% NFDM/TBST
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates with 5% NFDM/TBST
Lane 5 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates with 5% NFDM/TBST
Lane 6 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates with 5% NFDM/TBST
Lane 7 : Mouse liver lysates with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 53 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?

Exposure time: 50 seconds

The expression level of CD36 varies in different samples, and it could be upregulated by treatments such as PMA and Porphyromonas gingivalis (PMID: 8576181 and 27234131).

RAW 264.7 and mouse liver are reported to be positive for CD36 by PMID: 26187465 and 26186589, but this antibody failed to detect clear signal in normal conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (ab133625)

Ab133625 staining CD36 in paraffin embedded Human Hepatocellular cancer tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining on endothelial cells in human hepatocellular cancer.
Immunoprecipitation - Anti-CD36 antibody [EPR6573] (ab133625)

ab133625 (unpurified) at 1/5 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR6573] (ab133625)

Ab133625 staining CD36 in paraffin embedded Human cardiac muscle tissue sections by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). Tissue was counterstained with hematoxylin and heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/10,000 dilution (0.17µg/ml). A ready to use Goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Positive staining mainly on endothelial cells in human cardiac muscle.
Western blot - Anti-CD36 antibody [EPR6573] (ab133625)

All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution (unpurified)

Lane 1 : 3T3-L1 cell lysate
Lane 2 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 53 kDa
Observed band size: 78-88 kDa why is the actual band size different from the predicted?

The lysate in this image is prepared by 1%SDS Hot Lysate buffer. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
Western blot - Anti-CD36 antibody [EPR6573] (ab133625)

All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

Lane 1 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates prepared in RIPA lysis method with 5% NFDM/TBST
Lane 2 : 3T3-L1 (Mouse embryonic fibroblast) whole cell lysates prepared in 1%SDS Hot lysis method with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 53 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds
Western blot - Anti-CD36 antibody [EPR6573] (ab133625)

Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution + HEK293 (human embryonic kidney epithelial cell) transfected with His-tagged human CD36 (30aa-439aa) expression vector, whole cell lysate at 20 µg with 5% NFDM/TBST

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 53 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds
Western blot - Anti-CD36 antibody [EPR6573] (ab133625)

All lanes : Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution

Lane 1 : Human Heart Tissue Lysate
Lane 2 : Human Adipose Tissue Lysate
Lane 3 : Mouse Adipose Tissue Lysate
Lane 4 : Human Platelet Lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 53 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?

Exposure time: 2 minutes

This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133625 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Western blot - Anti-CD36 antibody [EPR6573] (ab133625)

Anti-CD36 antibody [EPR6573] (ab133625) at 1/10000 dilution (purified) + NIH/3T3 cell lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 78-88 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.

The lysate in this image is prepared by 1%SDS Hot lysis method.

For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
Western blot - Anti-CD36 antibody [EPR6573] (ab133625)

Anti-CD36 antibody [EPR6573] (ab133625) at 1/1000 dilution (unpurified) + NIH/3T3 at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 78-88 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.

The lysate in this image is prepared by 1%SDS Hot Lysate buffer. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
Immunoprecipitation - Anti-CD36 antibody [EPR6573] (ab133625)

ab133625 (purified) at 1/50 immunoprecipitating CD36 in 3T3-L1 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.
Anti-CD36 antibody [EPR6573] (ab133625)

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