Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CD36 antibody [EPR22512-58] (ab252923)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22512-58] to CD36
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-CD36 antibody [EPR22512-58]
See all CD36 primary antibodies
Description

Rabbit monoclonal [EPR22512-58] to CD36
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: U937 whole cell lysate; rat spleen tissue lysate; human placenta, lung and breast cancer tissue lysates. IHC-P: Human liver tissue; mouse spleen tissue; rat stomach tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22512-58
Isotype

IgG
Research areas

Western blot - Anti-CD36 antibody [EPR22512-58] (ab252923)

Anti-CD36 antibody [EPR22512-58] (ab252923) at 1/1000 dilution + Rat spleen tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 53 kDa

Exposure time: 81 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature(PMID:8798390,15677505, 20637247).
Western blot - Anti-CD36 antibody [EPR22512-58] (ab252923)

All lanes : Anti-CD36 antibody [EPR22512-58] (ab252923) at 1/1000 dilution

Lane 1 : Human placenta tissue lysate
Lane 2 : Human lung tissue lysate
Lane 3 : Human breast cancer tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 53 kDa
Observed band size: 70-110 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 5.5 seconds; Lane 2: 15 seconds; Lane 3: 48 seconds.

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247).
Western blot - Anti-CD36 antibody [EPR22512-58] (ab252923)

All lanes : Anti-CD36 antibody [EPR22512-58] (ab252923) at 1/1000 dilution

Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000 cells

Predicted band size: 53 kDa
Observed band size: 70-110 kDa why is the actual band size different from the predicted?

Exposure time: 58 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

Negative control: Ramos, Jurkat (PMID: 27956597, 10744776).

The molecular weight observed is due to glycosylation and consistent with what has been described in the literature (PMID:8798390,15677505,20637247,
8631821).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (ab252923)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling CD36 with ab252923 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of rat stomach (PMID:30047927, 22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (ab252923)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD36 with ab252923 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of mouse spleen (PMID:30047927, 22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [EPR22512-58] (ab252923)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CD36 with ab252923 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on endothelial cells of human liver (PMID:30047927, 22777768) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-CD36 antibody [EPR22512-58] (ab252923)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com