Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD36 antibody [FA6-152] (ab17044)

Key features and details

  • Mouse monoclonal [FA6-152] to CD36
  • Suitable for: IHC-P, Flow Cyt, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD36 antibody [FA6-152]
    See all CD36 primary antibodies

  • Description

    Mouse monoclonal [FA6-152] to CD36

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-Fr
    Human
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    20-weeks-old fetal erythrocytes.

  • Positive control

    • Flow cytometry: THP-1 cells; IHC-Fr: Human tonsil tissue; IHC-P: Human breast carcinoma tissue.

  • General notes

    We have data from customer abReviews and publications indicating that this antibody is capable of detecting CD36 via western blot. However, based on customer feedback of difficulties and extensive troubleshooting using this antibody in western blot, we are removing WB as a tested application and recommend ab133625 as an alternative for use in this application.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Frozen sections) - Anti-CD36 antibody [FA6-152] (ab17044)
    Immunohistochemistry (Frozen sections) - Anti-CD36 antibody [FA6-152] (ab17044)

    Immunohistochemistry of frozen section of human tonsil tissue stained for CD36 with ab17044 at 20 µg/ml.

  • Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)
    Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)

    Detection of CD36 in THP-1 cells. Red, black and blue line represent the isotype control, cells only and ab17044 at 10 μg/ml, respectively.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [FA6-152] (ab17044)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD36 antibody [FA6-152] (ab17044)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human breast carcinoma stained for CD36 with ab17044 at 20 µg/ml

  • Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)
    Flow Cytometry - Anti-CD36 antibody [FA6-152] (ab17044)
    Overlay histogram showing THP1 cells stained with ab17044 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab17044, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed THP1 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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