Anti-CD36 antibody (ab64014)
Key features and details
- Rabbit polyclonal to CD36
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CD36 antibody
See all CD36 primary antibodies -
Description
Rabbit polyclonal to CD36 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 50% homology with CD36/SCARB1 and CD36/SCARB2. Cross-reactivity with this protein has not been confirmed experimentally -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Images
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Western blot - Anti-CD36 antibody (ab64014)All lanes : Anti-CD36 antibody (ab64014) at 1 µg/ml
Lane 1 : Human Platelet (Human adult normal cell line) Whole Cell Lysate
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human adipose normal tissue lysate - total protein (ab28980)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
CD36 contains a number of potential glycosylation sites (SwissProt) which may explain the bands observed at 85 kDa. Abcam welcomes customer feedback. -
Western blot - Anti-CD36 antibody (ab64014)All lanes : Anti-CD36 antibody (ab64014) at 2 µg/ml (Blocked in 3% milk)
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lane 3 : Adult Mouse Brown Adipose Tissue Tissue Lysate
Lane 4 : Adult Rat Brown Adipose Tissue Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 53 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
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Western blot - Anti-CD36 antibody (ab64014)Anti-CD36 antibody (ab64014) + HEK293 (human embryonic kidney epithelial cell) transfected with His-tagged human CD36 (30aa-439aa) expression vector, whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 53 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
