Anti-Integrin beta 1 antibody (ab183666)
Key features and details
- Rabbit polyclonal to Integrin beta 1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Integrin beta 1 antibody
See all Integrin beta 1 primary antibodies -
Description
Rabbit polyclonal to Integrin beta 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB MouseRatHuman
-
Immunogen
Synthetic peptide within Human Integrin beta 1 aa 738-798. The exact sequence is proprietary.
Database link: P05556 -
Positive control
- A431, HeLa, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC12 and Rat-2 whole cell lysates/extracts. Human reast carcinoma and hepatoma tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Integrin beta 1 antibody (ab183666)All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/10000 dilution
Lane 1 : A431 whole cell lysate/extract
Lane 2 : HeLa whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (ab183666)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Integrin beta 1 with ab183666 at 1/500 dilution.
-
Western blot - Anti-Integrin beta 1 antibody (ab183666)All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/10000 dilution
Lane 1 : U87-MG whole cell lysate/extract
Lane 2 : SK-N-SH whole cell lysate/extract
Lane 3 : IMR32 whole cell lysate/extract
Lane 4 : SK-N-AS whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
-
Immunoprecipitation - Anti-Integrin beta 1 antibody (ab183666)ab183666 at 5μg immunoprecipitating Integrin beta 1 in HeLa whole cell extracts.
-
Western blot - Anti-Integrin beta 1 antibody (ab183666)All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/5000 dilution
Lane 1 : Neuro2A whole cell lysate/extract
Lane 2 : GL261 whole cell lysate/extract
Lane 3 : C8D30 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
-
Western blot - Anti-Integrin beta 1 antibody (ab183666)All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/5000 dilution
Lane 1 : NIH-3T3 whole cell lysate/extract
Lane 2 : BCL-1 whole cell lysate/extract
Lane 3 : Raw264.7 whole cell lysate/extract
Lane 4 : C2C12 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
-
Western blot - Anti-Integrin beta 1 antibody (ab183666)All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/5000 dilution
Lane 1 : PC-12 whole cell lysate/extract
Lane 2 : Rat-2 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody (ab183666)Immunohistochemical analysis of paraffin-embedded human Hepatoma tissue labeling Integrin beta 1 with ab183666 at 1/500 dilution.
