Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16895] to Integrin beta 1 - Low endotoxin, Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free
    See all Integrin beta 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR16895] to Integrin beta 1 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 and U-87 MG whole cell lysates; Human spleen lysate; Mouse heart and kidney; and Rat heart, kidney and spleen lysates. IHC-P: Human colon, Human hepatocellular carcinoma, rat stomach and mouse kidney tissues.

  • General notes

    ab221776 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

     

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: Integrin beta 1 knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: U87-MG cell lysate (20 µg)
    Lanes 4, 8 and 12: A431 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab179471 observed at 140 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab179471 was shown to react with Integrin beta 1 in wild-type HAP1 cells as well as additional cross-reactive bands. No bands were observed when Integrin beta 1 knockout samples were examined. Wild-type and Integrin beta 1 knockout samples were subjected to SDS-PAGE. ab179471 and ab8245 (loading control to GAPDH) were diluted 1/10,000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 1 with ab179471 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane staining on epithelial cells of Human colon is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Integrin beta 1 with ab179471 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane staining on tumor cells of Human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Integrin beta 1 knockout HAP1 cell lysate (20 µg)
    Lane 3: U87-MG cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab179471 observed at 140 kDa. Red signal from loading control – ab8245 observed at 37 kDa.

    This western blot image is a comparison between ab179471 and a competitor's top cited rabbit polyclonal antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)

    Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Integrin beta 1 with ab179471 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane staining on epithelial cells of rat stomach is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Integrin beta 1 with ab179471 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasmic staining on mouse kidney is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Anti-Integrin beta 1 antibody [EPR16895] (ab179471) at 1/20000 dilution + A431 (Human epidermoid carcinoma) whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Observed band size: 130-150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

     

  • Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Anti-Integrin beta 1 antibody [EPR16895] (ab179471) at 1/20000 dilution + U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Observed band size: 130-150 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

     

  • Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Anti-Integrin beta 1 antibody [EPR16895] (ab179471) at 1/2000 dilution + Human spleen lysates at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Observed band size: 130-150 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

     

  • Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Western blot - Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    All lanes : Anti-Integrin beta 1 antibody [EPR16895] (ab179471) at 1/2000 dilution

    Lane 1 : Mouse heart lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Rat heart lysates
    Lane 4 : Rat kidney lysates
    Lane 5 : Rat spleen lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Observed band size: 130-150 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179471).

     

  • Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)
    Anti-Integrin beta 1 antibody [EPR16895] - Low endotoxin, Azide free (ab221776)

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