Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14861] to Carbonic Anhydrase 12/CA12 - C-terminal
  • Suitable for: Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal
    See all Carbonic Anhydrase 12/CA12 primary antibodies

  • Description

    Rabbit monoclonal [EPR14861] to Carbonic Anhydrase 12/CA12 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7, SKOV3, 293, HeLa, A549, Human fetal kidney, Human ovary cancer and Human breast cancer lysates; Human renal adenocarcinoma, ovarian carcinoma, bladder transitional cell carcinoma, pancreas, colon and stomach tissues; SK-OV-3 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14861

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    All lanes : Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233) at 1/10000 dilution

    Lane 1 : MCF7 cell lysate
    Lanes 2 & 5 : SKOV3 cell lysate
    Lane 3 : 293 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 6 : Human fetal kidney lysate
    Lane 7 : Human ovary cancer lysate
    Lane 8 : Human breast cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 39 kDa


    Exposure time: 10 seconds
  • Flow Cytometry - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    Flow Cytometry - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

    Flow cytometric analysis of SK-OV-3 cells (paraformaldehyde-fixed, 2%) labeling CA12 with ab195233 at 1/130 dilution (red) or a Rabbit monoclonal IgG (negative) (black), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. Unlabeled cells (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and cytoplasm staining on human stomach tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and cytoplasm staining on human colon tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and weakly cytoplasm staining on human pancreas tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

    Immunohistochemical analysis of paraffin-embedded Human bladder transitional cell carcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and weakly cytoplasm staining on human transitional cell carcinoma of bladder tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling CA12 with ab195233 at 1/100  dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and weakly cytoplasm staining on human ovarian carcinoma tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

    Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CA12 with ab195233 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Cell membrane and cytoplasm staining on human renal adenocarcinoma tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)
    Anti-Carbonic Anhydrase 12/CA12 antibody [EPR14861] - C-terminal (ab195233)

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