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Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514)

Key features and details

  • Goat polyclonal to Carbonic Anhydrase 12/CA12
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Carbonic Anhydrase 12/CA12 antibody
    See all Carbonic Anhydrase 12/CA12 primary antibodies

  • Description

    Goat polyclonal to Carbonic Anhydrase 12/CA12

  • Host species

    Goat

  • Specificity

    ab223514 is expected to recognize all reported isoforms (NP_001209.1; NP_996808.1; NP_001280571.1).

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Carbonic Anhydrase 12/CA12 aa 150-250 (internal sequence) (Cysteine residue). (NP_001209.1).
    Database link: O43570

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Human kidney lysate. IHC-P: Human kidney tissue.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab223514 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514)

    Paraffin-embedded human kidney tissue stained for CA12 using ab223514 at 5 µg/ml in immunohistochemical analysis, followed by AP-staining.

  • Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514)
    Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514)
    Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514) at 0.3 µg/ml + human kidney lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

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