Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514)
Key features and details
- Goat polyclonal to Carbonic Anhydrase 12/CA12
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Carbonic Anhydrase 12/CA12 antibody
See all Carbonic Anhydrase 12/CA12 primary antibodies -
Description
Goat polyclonal to Carbonic Anhydrase 12/CA12 -
Host species
Goat -
Specificity
ab223514 is expected to recognize all reported isoforms (NP_001209.1; NP_996808.1; NP_001280571.1). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Carbonic Anhydrase 12/CA12 aa 150-250 (internal sequence) (Cysteine residue). (NP_001209.1).
Database link: O43570 -
Positive control
- WB: Human kidney lysate. IHC-P: Human kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223514 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514)
Paraffin-embedded human kidney tissue stained for CA12 using ab223514 at 5 µg/ml in immunohistochemical analysis, followed by AP-staining.
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Western blot - Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514)Anti-Carbonic Anhydrase 12/CA12 antibody (ab223514) at 0.3 µg/ml + human kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
