Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRMP2 antibody [EPR7792] (ab129082)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7792] to CRMP2
  • Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-CRMP2 antibody [EPR7792]
    See all CRMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR7792] to CRMP2

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    ICC/IF
    Mouse
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human CRMP2 (UniProt ID: Q16555).

  • Positive control

    • ICC/IF: NIH/3T3 cells. Flow Cyt: SH-SY5Y and NIH/3T3 cells. WB: PC-12, Jurkat, U-87 MG, HeLa and NIH/3T3 cell lysate. IHC-P: Human astrocytoma, Human colon carcinoma, and Human glioma tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Western blot - Anti-CRMP2 antibody [EPR7792] (ab129082)
    All lanes : Anti-CRMP2 antibody [EPR7792] (ab129082) at 1/10000 dilution

    Lane 1 : PC12 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : U87 MG cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 62 kDa
    Observed band size: 64 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR7792] (ab129082)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling CRMP2 with Purified ab129082 at 1:1000 dilution (0.12 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Flow Cytometry - Anti-CRMP2 antibody [EPR7792] (ab129082)

    Flow Cytometry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling CRMP2 with Purified ab129082 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR7792] (ab129082)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling CRMP2 with Purified ab129082 at 1:1000 dilution (0.12 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunoprecipitation - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Immunoprecipitation - Anti-CRMP2 antibody [EPR7792] (ab129082)

    ab129082 (purified) at 1:20 dilution (0.5µg) immunoprecipitating CRMP2 in U-87 MG whole cell lysate.
    Lane 1 (input): U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab129082 & U-87 MG whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129082 in U-87 MG whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Western blot - Anti-CRMP2 antibody [EPR7792] (ab129082)
    All lanes : Anti-CRMP2 antibody [EPR7792] (ab129082) at 1/50000 dilution

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 62 kDa
    Observed band size: 64 kDa why is the actual band size different from the predicted?



    U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates 

  • Western blot - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Western blot - Anti-CRMP2 antibody [EPR7792] (ab129082)
    All lanes : Anti-CRMP2 antibody [EPR7792] (ab129082) at 1/100000 dilution

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysates

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 62 kDa
    Observed band size: 64 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 antibody [EPR7792] (ab129082)

    ab129082 staining CRMP2 in NIH/3T3 (mouse embryonic fibroblast) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1: PBS only.

  • Flow Cytometry - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Flow Cytometry - Anti-CRMP2 antibody [EPR7792] (ab129082)

    Overlay histogram showing SH-SY5Y cells stained with ab129082 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129082, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 antibody [EPR7792] (ab129082)

    ab129082, at a dilution of 1/100, staining CRMP2 in paraffin embedded Human astrocytoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-CRMP2 antibody [EPR7792] (ab129082)
    OI-RD Scanning - Anti-CRMP2 antibody [EPR7792] (ab129082)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CRMP2 antibody [EPR7792] (ab129082)
    Anti-CRMP2 antibody [EPR7792] (ab129082)

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