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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-CRMP2 antibody [1B1] (ab62539)

Anti-CRMP2 antibody [1B1] (ab62539)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [1B1] to CRMP2
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-CRMP2 antibody [1B1]
    See all CRMP2 primary antibodies
  • Description

    Mouse monoclonal [1B1] to CRMP2
  • Host species

    Mouse
  • Specificity

    Does not react with CRMP-1, CRMP-3, CRMP-4 or CRMP-5.

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Immunogen: A 15 residue synthetic peptide derived from C-terminal domain of human collapsing-response mediator protein Type 2 (MW: 65 kDa).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 1% Sucrose, 1.21% Tris, 0.375% Glycine
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    1B1
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis

Images

  • Western blot - Anti-CRMP2 antibody [1B1] (ab62539)
    Western blot - Anti-CRMP2 antibody [1B1] (ab62539)
    Anti-CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 65 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-CRMP2 antibody [1B1] (ab62539)
    Flow Cytometry - Anti-CRMP2 antibody [1B1] (ab62539)
    Overlay histogram showing SH-SY5Y cells stained with ab62539 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62539, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CRMP2 antibody [1B1] (ab62539)

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    Anti-CRMP2 antibody [EPR7792] (ab129082)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Alexa Fluor® 647 Anti-CRMP2 antibody [EPR7792] (ab204666)

    Applications: ICC/IF

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    Anti-CRMP2 (phospho T514) antibody [EPR7876] (ab129066)

    Applications: WB

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    Anti-CRMP2 antibody [EPR7792] - BSA and Azide free (ab240952)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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