Anti-CRMP2 antibody [1B1] (ab62539)
Key features and details
- Mouse monoclonal [1B1] to CRMP2
- Suitable for: Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-CRMP2 antibody [1B1]
See all CRMP2 primary antibodies -
Description
Mouse monoclonal [1B1] to CRMP2 -
Host species
Mouse -
Specificity
Does not react with CRMP-1, CRMP-3, CRMP-4 or CRMP-5.
-
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Immunogen: A 15 residue synthetic peptide derived from C-terminal domain of human collapsing-response mediator protein Type 2 (MW: 65 kDa).
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 1% Sucrose, 1.21% Tris, 0.375% Glycine -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1B1 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
-
Anti-CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 65 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
-
Overlay histogram showing SH-SY5Y cells stained with ab62539 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62539, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.