Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD73 using ab257309 at 2 μg/ml in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for CD73 using ab257309 at 2 μg/ml in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for CD73 using ab257309 at 2 μg/ml in immunohistochemical analysis.

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CD73 using ab257309 at 2 μg/ml in immunohistochemical analysis.

Immunfluorescence staining of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells stained for CD73 using ab257309 at 2 μg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).

Flow Cytometric Analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labeling CD73 using ab257309 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

All lanes : Anti-CD73 antibody [NT5E/2503] (ab257309) at 2 µg/ml

Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 2 : PC3 (human prostate adenocarcinoma cell line) cell lysate

Predicted band size: 63 kDa

Analysis of Protein Array containing more than 19,000 full-length human proteins using ab257309.

Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.