Anti-CRMP2 (phospho T514) antibody (ab62478)
Key features and details
- Rabbit polyclonal to CRMP2 (phospho T514)
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CRMP2 (phospho T514) antibody
See all CRMP2 primary antibodies -
Description
Rabbit polyclonal to CRMP2 (phospho T514) -
Host species
Rabbit -
Specificity
ab62478 detects endogenous levels of CRMP2 only when phosphorylated at threonine 514. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide derived from human CRMP2 around the phosphorylation site of threonine 514 (T-V-TP-P-A).
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab62478 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Anti-CRMP2 (phospho T514) antibody (ab62478) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP2 (phospho T514) antibody (ab62478)ab62478, at 1/50 dilution, staining CRMP2 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
-
ab62478, at 1/500 dilution, staining CRMP2 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
-
Immunocytochemistry/ Immunofluorescence - Anti-CRMP2 (phospho T514) antibody (ab62478) Image from Ou Y et al., PLoS ONE 7(11): e48773. Fig 5.; doi: 10.1371/journal.pone.0048773. Epub 2012 Nov 21.
Immunofluorescence analysis of RPE (human retinal pigmented epithelium) cells, staining CRMP2 (phospho T514) with ab62478. Cells were fixed in cold methanol and incubated with primary antibody, followed by an AlexaFluor®488-conjugated anti rabbit IgG.