Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ITPK1 antibody [EPR10631] (ab175222)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10631] to ITPK1
Suitable for: IHC-P, WB, IP, Flow Cyt
Reacts with: Human

Product name

Anti-ITPK1 antibody [EPR10631]
See all ITPK1 primary antibodies
Description

Rabbit monoclonal [EPR10631] to ITPK1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ITPK1 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: Q13572
Positive control
- Fetal brain, fetal heart, HepG2 and Hela lysates; Human brain and liver tissues; Hela cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10631
Isotype

IgG
Research areas

Immunoprecipitation - Anti-ITPK1 antibody [EPR10631] (ab175222)

Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175222 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Flow Cytometry - Anti-ITPK1 antibody [EPR10631] (ab175222)

Flow cytometric analysis of permeabilized HeLa cells labeling ITPK1 with ab175222 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITPK1 with ab175222 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ITPK1 with ab175222 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-ITPK1 antibody [EPR10631] (ab175222)

All lanes : Anti-ITPK1 antibody [EPR10631] (ab175222) at 1/10000 dilution

Lane 1 : Fetal brain lysate
Lane 2 : Fetal liver lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Hela cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 46 kDa
Anti-ITPK1 antibody [EPR10631] (ab175222)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com