Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ITPK1 antibody [EPR10631] (ab175222)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10631] to ITPK1
  • Suitable for: IHC-P, WB, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ITPK1 antibody [EPR10631]
    See all ITPK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10631] to ITPK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ITPK1 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q13572

  • Positive control

    • Fetal brain, fetal heart, HepG2 and Hela lysates; Human brain and liver tissues; Hela cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10631

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-ITPK1 antibody [EPR10631] (ab175222)
    Immunoprecipitation - Anti-ITPK1 antibody [EPR10631] (ab175222)

    Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175222 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Flow Cytometry - Anti-ITPK1 antibody [EPR10631] (ab175222)
    Flow Cytometry - Anti-ITPK1 antibody [EPR10631] (ab175222)

    Flow cytometric analysis of permeabilized HeLa cells labeling ITPK1 with ab175222 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITPK1 with ab175222 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPK1 antibody [EPR10631] (ab175222)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ITPK1 with ab175222 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-ITPK1 antibody [EPR10631] (ab175222)
    Western blot - Anti-ITPK1 antibody [EPR10631] (ab175222)
    All lanes : Anti-ITPK1 antibody [EPR10631] (ab175222) at 1/10000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 46 kDa

  • Anti-ITPK1 antibody [EPR10631] (ab175222)
    Anti-ITPK1 antibody [EPR10631] (ab175222)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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