Mouse TFPI ELISA Kit (ab217776)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.7 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TFPI ELISA Kit
See all TFPI kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Mouse serum 8 3.7% Inter-assay Sample n Mean SD CV% Mouse serum 3 7.2% -
Sample type
Cell culture supernatant, Urine, Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.7 pg/ml -
Range
18.75 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 99 97% - 102% Serum 102 96% - 109% Tissue Extracts 92 85% - 101% Cell culture media 98 96% - 101% Hep Plasma 109 104% - 112% EDTA Plasma 101 96% - 105% Cit plasma 103 98% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse TFPI ELISA Kit (ab217776) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TFPI protein in cit plasma, edta plasma, hep plasma, serum, tissue extracts, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TFPI with 1.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse TFPI (tissue factor pathway inhibitor) is a secreted protein located on the endothelial surface, within platelets, in plasma, and on monocytes. Functionally, mouse TFPI is the primary inhibitor of intravascular tissue factor (TF) activity. TF is the principal protein that initiates blood coagulation in vivo. Thus, Mouse TFPI inhibits coagulation.
Mouse TFPI has an acidic N-terminal region, three Kunitz (K) domains separated by two linker regions, and a C-terminal basic region. The first K domain inhibits TF from binding coagulation factor VIIa. The second K domain inhibits factor Xa. The third K domain binds to heparin. Human and rat TFPI exhibit 58% and 71% amino acid identity to mouse TFPI, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests Mouse TFPI Lyophilized Recombinant Protein 2 vials 10X Mouse TFPI Detector Antibody 1 x 600µl 10X Mouse TFPI Capture Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
Tissue specificity
Mostly in endothelial cells. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Anti convertin
- EPI
- Extrinsic pathway inhibitor
see all -
Database links
- Entrez Gene: 21788 Mouse
- SwissProt: O54819 Mouse
- Unigene: 124316 Mouse
- Unigene: 447013 Mouse
Images
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Other - Mouse TFPI ELISA Kit (ab217776)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse TFPI standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse TFPI standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TFPI in mouse serum, and plasma samples.The concentrations of TFPI were measured in duplicates, interpolated from the TFPI standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100, plasma (citrate) 1:400, plasma (EDTA) 1:400 and plasma (heparin) 1:400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 57.10 ng/mL in neat serum, 241.24 ng/mL in neat plasma (citrate), 259.59 ng/mL in neat plasma (EDTA) and 303.08 ng/mL in neat plasma (heparin).
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Interpolated concentrations of native and spiked TFPI in mouse urine and spiked TFPI in Cell Culture Media samples.The concentrations of TFPI were measured in duplicates, interpolated from the TFPI standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 50%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 120.64 pg/mL in native, neat urine, 1,353.96 pg/mL in spiked, neat urine and 1,130.58 pg/mL in spiked, neat cell culture media.
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Interpolated concentrations of native TFPI in mouse heart and liver tissue extract samples based on a 500 µg/mL extract load.The concentrations of TFPI were measured in duplicate and interpolated from the TFPI standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI concentration was determined to be 525.44 pg/mL in mouse heart extract and 69.55 pg/mL in mouse liver extract.
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Sandwich ELISA - Mouse TFPI ELISA Kit (ab217776)To learn more about the advantages of recombinant antibodies see here.
