Monkey IL-2 ELISA Kit (ab119541)
Key features and details
- Sensitivity: 10 U/ml
- Range: 20 U/ml - 1250 U/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey
Overview
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Product name
Monkey IL-2 ELISA Kit
See all IL-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 Inter-assay Sample n Mean SD CV% Overall 8 -
Sample type
Cell culture supernatant, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
10 U/ml -
Range
20 U/ml - 1250 U/ml -
Recovery
80 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Monkey -
Product overview
Abcam’s IL-2 Monkey in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Monkey IL-2 concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, urine and whole blood.
IL-2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated IL-2 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after the addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-2 captured on the plate.
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Notes
Standard is recombinant protein produced in E.coli.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-monkey IL-2 polyclonal antibody 1 x 70µl Microplate coated with monoclonal antibody to monkey IL-2 1 unit Monkey IL-2 Standard lyophilized 2 vials Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
Research areas
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Function
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
Involvement in disease
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
Sequence similarities
Belongs to the IL-2 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Aldesleukin
- IL 2
- IL-2
see all
Images
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Typical Standard Curve
Representative Standard Curve using ab119541.
