Mouse TFPI Matched Antibody Pair Kit (ab222259)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 15.6 pg/ml - 1000 pg/ml
Overview
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Product name
Mouse TFPI Matched Antibody Pair Kit
See all TFPI kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
6.2 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse TFPI Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse TFPI.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab217776. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse TFPI Capture Antibody 2 x 50µg 1 x 50µg Mouse TFPI Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse TFPI Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. -
Tissue specificity
Mostly in endothelial cells. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Anti convertin
- EPI
- Extrinsic pathway inhibitor
see all -
Database links
- Entrez Gene: 21788 Mouse
- SwissProt: O54819 Mouse
- Unigene: 124316 Mouse
- Unigene: 447013 Mouse
Images
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Mouse TFPI Standard Curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse TFPI Matched Antibody Pair Kit (ab222259)To learn more about the advantages of recombinant antibodies see here.
