Human ALDH1A1 ELISA Kit (ab214024)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 220 pg/ml
- Range: 1.25 ng/ml - 80 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ALDH1A1 ELISA Kit
See all ALDH1A1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% A549 extract 8 4.8% Inter-assay Sample n Mean SD CV% A549 extract 3 5.5% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
220 pg/ml -
Range
1.25 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 76 73% - 83% Tissue Extracts 109 109% - 110% Cell culture media 108 105% - 110% Hep Plasma 75 70% - 80% EDTA Plasma 86 78% - 96% Cit plasma 74 68% - 80% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human ALDH1A1 ELISA Kit (ab214024) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ALDH1A1 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human ALDH1A1 with 220 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
ALDH1A1 belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase follows alcohol dehydrogenase in the major pathway of alcohol metabolism. Notably, there are two major aldehyde dehydrogenase isozymes in the liver: cytosolic and mitochondrial. This gene encodes the cytosolic isozyme. Finally, human ALDH1A1 is 501 amino acids in length. Mouse and rat ALDH1A1 are 87% and 86% identical to human ALDH1A1, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human ALDH1A1 Capture Antibody 1 x 600µl 10X Human ALDH1A1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human ALDH1A1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
Pathway
Cofactor metabolism; retinol metabolism. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Acetaldehyde dehydrogenase 1
- AHD2
- AL1A1_HUMAN
see all -
Database links
- Entrez Gene: 216 Human
- Omim: 100640 Human
- SwissProt: P00352 Human
- Unigene: 76392 Human
Images
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Other - Human ALDH1A1 ELISA Kit (ab214024)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human ALDH1A1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human ALDH1A1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native ALDH1A1 in human serum and plasma samples.The concentrations of ALDH1A1 were measured in duplicates, interpolated from the ALDH1A1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 50%, plasma (heparin) 50%, and plasma (EDTA) 50%. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALDH1A1 concentration was determined to be 4.38 ng/ml in serum, 7.37 ng/ml in plasma (citrate), 11.12 ng/ml in plasma (heparin), and 14.40 ng/ml in plasma (EDTA).
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Interpolated concentrations of native ALDH1A1 in human A549, Hep G2, fetal brain, and liver homogenate extract samples.Based on 25 μg/mL, 20 μg/mL, 100 μg/mL, and 1.25 μg/mL extract loads, respectively. The concentrations of ALDH1A1 were measured in duplicate and interpolated from the ALDH1A1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALDH1A1 concentration was determined to be 59.4 ng/mL, 76.8 ng/mL, 13.1 ng/mL, and 10.5 ng/mL in A549, Hep G2, fetal brain, and liver homogenate extract samples, respectively.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ALDH1A1 concentration was determined to be 18.98 ng/mL with a range of 1.72 – 143.05 ng/mL.
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Sandwich ELISA - Human ALDH1A1 ELISA Kit (ab214024)To learn more about the advantages of recombinant antibodies see here.
