Human ALDH1A1 Matched Antibody Pair Kit (ab218175)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 1.25 ng/ml - 80 ng/ml
Overview
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Product name
Human ALDH1A1 Matched Antibody Pair Kit
See all ALDH1A1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
289.4 pg/ml -
Range
1.25 ng/ml - 80 ng/ml -
Species reactivity
Reacts with: Human -
Product overview
Human ALDH1A1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human ALDH1A1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214024. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human ALDH1A1 Capture Antibody 2 x 50µg 1 x 50µg Human ALDH1A1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human ALDH1A1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Binds free retinal and cellular retinol-binding protein-bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. -
Pathway
Cofactor metabolism; retinol metabolism. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Acetaldehyde dehydrogenase 1
- AHD2
- AL1A1_HUMAN
see all -
Database links
- Entrez Gene: 216 Human
- Omim: 100640 Human
- SwissProt: P00352 Human
- Unigene: 76392 Human
Images
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Standard calibration curve. Background subtracted values are graphed.
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To learn more about the advantages of recombinant antibodies see here.