Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11787(B)] to PP2A alpha + beta
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
Overview
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Product name
Anti-PP2A alpha + beta antibody [EPR11787(B)]
See all PP2A alpha + beta primary antibodies -
Description
Rabbit monoclonal [EPR11787(B)] to PP2A alpha + beta -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 100% homology with PP2A beta. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB HumanRecombinant fragment -
Immunogen
Synthetic peptide corresponding to Human PP2A alpha + beta.
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Positive control
- HeLa, 293T, A431 and NIH/3T3 cell lysates; A431 cells; Immunoprecipitation pellet from HeLa cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11787(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution
Lane 1 : Human PP2A beta full length recombinant protein
Lane 2 : Human PP2A alpha full length recombinant protein
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and Diluting buffer and concentration: 5% NFDM/TBST.
Human PP2A beta full length recombinant protein contains aa1-309 with a GST-Tag (catalog#: ab128562).
Human PP2A alpha full length recombinant protein contains aa1-309 with a GST-Tag (catalog#: ab128557).
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All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDa
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Immunofluorescent analysis of A431 cells labeling PP2A alpha + beta with ab168350 at 1/50 dilution.
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Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/10000 dilution + immunoprecipitation pellet from HeLa cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 36 kDa
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