Anti-PP2A alpha + beta antibody [Y119] (ab32141)
![Anti-PP2A alpha + beta antibody [Y119] (ab32141)](https://www.abcam.com/ps/products/32/ab32141/Images/ab32141-44016-anti-pp2a-alpha-beta-antibody-y119-western-blot.jpg "Anti-PP2A alpha + beta antibody [Y119] (ab32141)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y119] to PP2A alpha + beta
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-PP2A alpha + beta antibody [Y119]
See all PP2A alpha + beta primary antibodies -
Description
Rabbit monoclonal [Y119] to PP2A alpha + beta -
Host species
Rabbit -
Specificity
This antibody detects both alpha and beta isoforms of PP2A. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Epitope
ab32141 reacts with an epitope located in the C terminal region of PP2A alpha and beta -
Positive control
- T47-D cell lysate and human breast carcinoma
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y119 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PP2A alpha + beta antibody [Y119] (ab32141)Anti-PP2A alpha + beta antibody [Y119] (ab32141) at 1/10000 dilution + T47-D cell lysate
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)](https://www.abcam.com/ps/products/32/ab32141/Images/ab32141-44015-anti-pp2a-alpha-beta-antibody-y119-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [Y119] (ab32141)ab32141,at a 1/250 dilution, staining human breast carcinoma by immunohistochemistry, Paraffin embedded tissue
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-PP2A alpha + beta antibody [Y119] (ab32141)](https://www.abcam.com/ps/products/32/ab32141/Images/ab32141-270741-anti-pp2a-alpha-beta-antibody-y119-flow-cytometry.jpg)
Flow Cytometry - Anti-PP2A alpha + beta antibody [Y119] (ab32141)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling PP2A alpha + beta (red) with unpurified ab32141 at dilution of 1/20. Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary and secondary antibodies.
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![Anti-PP2A alpha + beta antibody [Y119] (ab32141)](https://www.abcam.com/ps/products/32/ab32141/Images/ab32141-4-benefits-of-recombinant-antibodies.png)
Anti-PP2A alpha + beta antibody [Y119] (ab32141)
