Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597)
Key features and details
- Mouse monoclonal [2A10] to PP2A alpha + beta (methyl L309)
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-PP2A alpha + beta (methyl L309) antibody [2A10]
See all PP2A alpha + beta primary antibodies -
Description
Mouse monoclonal [2A10] to PP2A alpha + beta (methyl L309) -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Saccharomyces cerevisiae -
Immunogen
Synthetic peptide corresponding to Human PP2A alpha + beta conjugated to keyhole limpet haemocyanin. A KLH-coupled peptide with the sequence: KLH-C-ßA-RRTPDYFL-OMe. The c-terminal sequence (as well as the carboxy-methylation) is absolutely conserved from human, mouse to yeast.
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Positive control
- This antibody gave a positive signal in Hek293 and PC12 whole cell lysates
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
2A10 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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All lanes : Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597) at 10 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 20 minutes
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Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597) at 1/100 dilution + Lystae prepared from mouse embryonic fibroblast cells at 25 µg
Secondary
HRP-conjugatedsheep polyclonal to mouse IgG at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
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All lanes : Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597) at 1/1000 dilution
Lane 1 : No LCMT1 with 100% Licor Blocking Buffer for 30 minutes at 25°C.
Lane 2 : LCMT1 at 0.02 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.
Lane 3 : LCMT1 at 0.1 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.
Lane 4 : LCMT1 at 0.5 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.
Secondary
All lanes : IRDye 800CW conjugated goat anti-mouse polyclonal at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
Detection method: Licor 10 minute exposure.
Specific observed bands at 40kDa