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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597)

Price and availability

281 433 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [2A10] to PP2A alpha + beta (methyl L309)
  • Suitable for: WB
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PP2A alpha + beta (methyl L309) antibody [2A10]
    See all PP2A alpha + beta primary antibodies
  • Description

    Mouse monoclonal [2A10] to PP2A alpha + beta (methyl L309)
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to Human PP2A alpha + beta conjugated to keyhole limpet haemocyanin. A KLH-coupled peptide with the sequence: KLH-C-ßA-RRTPDYFL-OMe. The c-terminal sequence (as well as the carboxy-methylation) is absolutely conserved from human, mouse to yeast.

  • Positive control

    • This antibody gave a positive signal in Hek293 and PC12 whole cell lysates
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    2A10
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Troponin

Images

  • Western blot - Anti-PP2A alpha + beta (methylation L309) antibody [2A10] (ab66597)
    Western blot - Anti-PP2A alpha + beta (methylation L309) antibody [2A10] (ab66597)
    All lanes : Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597) at 10 µg/ml

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa


    Exposure time: 20 minutes
  • Western blot - Anti-PP2A alpha + beta (methylation L309) antibody [2A10] (ab66597)
    Western blot - Anti-PP2A alpha + beta (methylation L309) antibody [2A10] (ab66597) This image is a courtesy of Anonymous Abreview
    Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597) at 1/100 dilution + Lystae prepared from mouse embryonic fibroblast cells at 25 µg

    Secondary
    HRP-conjugatedsheep polyclonal to mouse IgG at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute

    See Abreview

  • Western blot - Anti-PP2A alpha + beta (methylation L309) antibody [2A10] (ab66597)
    Western blot - Anti-PP2A alpha + beta (methylation L309) antibody [2A10] (ab66597) This image is courtesy of an anonymous Abreview
    All lanes : Anti-PP2A alpha + beta (methyl L309) antibody [2A10] (ab66597) at 1/1000 dilution

    Lane 1 : No LCMT1 with 100% Licor Blocking Buffer for 30 minutes at 25°C.
    Lane 2 : LCMT1 at 0.02 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.
    Lane 3 : LCMT1 at 0.1 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.
    Lane 4 : LCMT1 at 0.5 µg/ml with 100% Licor Blocking Buffer for 30 minutes at 25°C.

    Secondary
    All lanes : IRDye 800CW conjugated goat anti-mouse polyclonal at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa



    Detection method: Licor 10 minute exposure.
    Specific observed bands at 40kDa

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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