Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

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Anti-PP2A alpha + beta antibody [YE351] (ab32065)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [YE351] to PP2A alpha + beta
Suitable for: IP, WB, IHC-P, Dot blot
Reacts with: Mouse, Rat, Human

Product name

Anti-PP2A alpha + beta antibody [YE351]
See all PP2A alpha + beta primary antibodies
Description

Rabbit monoclonal [YE351] to PP2A alpha + beta
Host species

Rabbit
Specificity

This antibody can bind both alpha and beta form of PP2A. Mouse samples have been successfully tested in WB, but IHC-P sections from various mouse tissues showed negative results.

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human PP2A alpha + beta (N terminal). The exact sequence is proprietary.
Epitope

ab32065 reacts with an epitope located in the N terminal region of PP2A alpha.
Positive control
- WB: A431 cell lysate and NIH 3T3 cell lysate. Dot Blot: PP2A-alpha peptide and PP2A-beta peptide.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

YE351
Isotype

IgG
Research areas

Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

All lanes : Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/50000 dilution

Lane 1 : A431 whole cell lysate
Lane 2 : A549 whole cell lysate
Lane 3 : Caco-2 whole cell lysate
Lane 4 : T47D whole cell lysate
Lane 5 : C2C12 whole cell lysate
Lane 6 : HeLa whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 15 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

All lanes : Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/50000 dilution

Lane 1 : Jurkat whole cell lysate
Lane 2 : U-937 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 5 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

All lanes : Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/100000 dilution

Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : Human fetal spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 30 seconds

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/100000 dilution + Human fetal brain tissue lysate at 10 µg

Secondary
HRP-conjugate anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 15 seconds

Blocking and dilution buffer: 5% NFDM /TBST.
Western blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

All lanes : Anti-PP2A alpha + beta antibody [YE351] (ab32065) at 1/100000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lane 9 : C6 whole cell lysate
Lane 10 : Raw264.7 whole cell lysate
Lane 11 : PC-12 whole cell lysate
Lane 12 : NIH/3T3 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Exposure time: 1 second

Blocking and dilution buffer: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Cytoplasm and weak nucleus staining is visible in lymphocytes of human tonsil.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Cytoplasm staining is visible in epithelial cells of mouse kidney
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Cytoplasm and weak nucleus staining is visible in epithelial cells of rat kidney.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse heart tissue labelling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Cytoplasm staining is visible in mouse heart.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat heart tissue labelling PP2A alpha + beta with ab32065 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Cytoplasm and nucleus staining is visible on rat heart.
Immunoprecipitation - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

ab32065 at 1/80 immunoprecipitating PP2A alpha + beta in HeLa whole cell lysate.

Lane 1 (input): HeLa whole cell lysate (10µg)

Lane 2 (+): ab32065 + HeLa whole cell lysate.

Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32065 in HeLa (human cervix adenocarcinoma) whole cell lysate.

For western blotting, ab32065 (1/500) ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/10000).

Blocking buffer and concentration: 5% NFDM/TBST.

Diluting buffer and concentration: 5% NFDM /TBST.

Observed band: 37kDa.
Dot Blot - Anti-PP2A alpha + beta antibody [YE351] (ab32065)

Dot blot analysis of PP2A-alpha peptide (Lane 1) and PP2A-beta peptide (Lane 2) labelling PP2A alpha+beta with ab32065 at a dilution of 1/1000. A HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody at a dilution of 1/1000.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-PP2A alpha + beta antibody [YE351] (ab32065)

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