Alkaline Phosphatase Assay Kit (Luminometric) (ab233466)
Key features and details
- Assay type: Quantitative
- Detection method: Luminescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Alkaline Phosphatase Assay Kit (Luminometric)
See all Alkaline Phosphatase kits -
Detection method
Luminescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Quantitative -
Product overview
Alkaline Phosphatase Assay Kit (ab233466) uses D-luciferin phosphate as the luminogenic phosphatase substrate to quantify alkaline phosphatase activity in solutions and cells.
D-luciferin phosphate is not recognized by luciferase until its phosphate group is removed to give luciferin. This kit provides all the essential components with an optimized assay protocol which is compatible with HTS liquid-handling instruments. The assay can be readily performed in a 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.
The high sensitivity makes the kit ideal for the assays that require low detection limit.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Alkaline Phosphatase Standard 1 vial Assay Buffer 1 x 5ml Phosphatase Substrate 1 vial Reaction Buffer 1 x 5ml -
Research areas
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Sequence similarities
Belongs to the alkaline phosphatase family. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Alkaline phosphatase, germ cell
- Alkaline phosphatase, testicular and thymus
- Akp2
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Images
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Example Standard Curve
Alkaline phosphatase dose response was measured with Alkaline Phosphatase Assay Kit (Luminometric) (ab233466) in a solid white 96-well plate using a NovoStar microplate reader (BMG Labtech). As low as 0.001 mU/mL alkaline phosphatase can be detected with 20 minutes incubation (n=3).
