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Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)

Key features and details

  • Donkey Anti-Mouse IgG H&L (DyLight® 550)
  • Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB

Overview

  • Product name

    Donkey Anti-Mouse IgG H&L (DyLight® 550)
    See all IgG secondary antibodies

  • Host species

    Donkey

  • Target species

    Mouse

  • Specificity

    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details

  • Conjugation

    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • General notes

    DyLight® 550 replaces DyLight® 549.

  • Research areas

Images

  • Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876)
    Western blot - Donkey Anti-Mouse IgG H&L (DyLight® 550) (ab96876) Courtesy of an anonymous Abreview
    All lanes : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) at 1/1000 dilution
    1. Lane 1 : Rat Hippocampus
    2. Lane 2 : Rat Hippocampus
    3. Lane 3 : Rat Hippocampus

    Lysates/proteins at 25 µg per lane.

    Secondary:

    1. Lane 1 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/5000 dilution
    2. Lane 2 : Rabbit polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 550), pre-adsorbed (ab98786) at 1/1000 dilution
    3. Lane 3 : Other Commercial Dye at 1/1000 dilution

      4. Performed under reducing conditions.

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