HRP Anti-Cdc25C antibody [E302] (ab205734)
![HRP Anti-Cdc25C antibody [E302] (ab205734)](https://www.abcam.com/ps/products/205/ab205734/Images/ab205734-270792-WBab2057341.jpg "HRP Anti-Cdc25C antibody [E302] (ab205734)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [E302] to Cdc25C
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-Cdc25C antibody [E302]
See all Cdc25C primary antibodies -
Description
HRP Rabbit monoclonal [E302] to Cdc25C -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human Cdc25C (N terminal). The exact sequence is proprietary.
Database link: P30307 -
Positive control
- WB: HeLa and wild-type HAP1 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E302 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-Cdc25C antibody [E302] (ab205734)All lanes : HRP Anti-Cdc25C antibody [E302] (ab205734) at 1/2500 dilution
Lane 1 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : Wild-type HAP1 cell lysate at 20 µg
Lane 3 : Cdc25C knockout HAP1 cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab205734 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-Cdc25C antibody [E302] (ab205734)](https://www.abcam.com/ps/products/205/ab205734/Images/ab205734-3-benefits-of-recombinant-antibodies.png)
HRP Anti-Cdc25C antibody [E302] (ab205734)
