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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-Cdc25C antibody (ab226958)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Cdc25C antibody (ab226958)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Cdc25C
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cdc25C antibody
    See all Cdc25C primary antibodies
  • Description

    Rabbit polyclonal to Cdc25C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Pig, Chimpanzee
  • Immunogen

    Recombinant fragment within Human Cdc25C (internal sequence). The exact sequence is proprietary.
    Database link: P30307

  • Positive control

    • WB: RAW 264.7, Raji, PC-12, Rat2 and C2C12 whole cell extracts. IHC-P: Human SAS xenograft tissue. ICC/IF: U-2 OS cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases

Images

  • Western blot - Anti-Cdc25C antibody (ab226958)
    Western blot - Anti-Cdc25C antibody (ab226958)
    All lanes : Anti-Cdc25C antibody (ab226958) at 1/500 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.


    10% SDS-PAGE gel.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cdc25C antibody (ab226958)
    Immunocytochemistry/ Immunofluorescence - Anti-Cdc25C antibody (ab226958)

    PFA-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Cd25C (green) using ab226958 at 1/500 dilution in ICC/IF. Counterstain: alpha-tubulin filaments were labeled with an anti-alpha Tubulin antibody (red) at 1/2000 dilution.

  • Western blot - Anti-Cdc25C antibody (ab226958)
    Western blot - Anti-Cdc25C antibody (ab226958)
    Anti-Cdc25C antibody (ab226958) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg


    10% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C antibody (ab226958)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25C antibody (ab226958)

    Paraffin-embedded human SAS xenograft tissue stained for Cdc25C using ab226958 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-Cdc25C antibody (ab226958)
    Western blot - Anti-Cdc25C antibody (ab226958)
    All lanes : Anti-Cdc25C antibody (ab226958) at 1/1000 dilution

    Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : C2C12 (mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.


    10% SDS-PAGE gel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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