Acid Phosphatase Assay Kit (Fluorometric) (ab83370)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.001 mU/ml
Overview
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Product name
Acid Phosphatase Assay Kit (Fluorometric)
See all Acid phosphatase kits -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Enzyme activity -
Sensitivity
0.001 mU/ml -
Product overview
Abcam's Acid Phosphatase Assay Kit (Fluorometric) uses non-fluorescent Methylumbelliferyl phosphate disodium (MUP) as the substrate which has Ex/Em=360/440 nm when dephosphorylated by AP. The kit is an ultra-sensitive, simple, direct and HTS-ready fluorometric assay designed to measure AP activity in serum and other bio-samples.
Visit our FAQs page for tips and troubleshooting. -
Notes
The detection sensitivity is ~1 µU, more sensitive than colorimetric assays.
The kit is suitable for both research and drug discovery.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 500 tests AP Assay buffer NM 1 x 100ml AP Enzyme (Lyophilised) Green 1 vial MUP Substrate Red 1 vial Stop Solution WM 1 x 25ml -
Research areas
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Relevance
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease. -
Cellular localization
ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein. -
Alternative names
- Acid phosphatase 1 soluble
- Acid phosphatase 2 lysosomal
- Acid phosphatase 5 tartrate resistant
see all
Images
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Functional Studies - Acid Phosphatase Fluorometric Assay Kit (ab83370)4-Methylumbelliferone (4-MU) Standard Curve.
