LysoLive™ Lysosomal Phosphatase Assay Kit (ab253378)
Key features and details
- Detection method: Fluorescent
Overview
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Product name
LysoLive™ Lysosomal Phosphatase Assay Kit
See all Acid phosphatase kits -
Detection method
Fluorescent -
Product overview
The MarkerGene™LysoLive™ Lysosomal Phosphatase Assay Kit utilizes the sensitive substrate PhosGreen™ to monitor phosphatase activity in the lysosomes of live cells.
Lysosomes are acidic cytoplasmic organelles that are present in all nucleated mammalian cells. Lysosomes have been found to be involved in a variety of cellular processes including repair of the plasma membrane, defense against pathogens, cholesterol homeostasis, bone remodeling, metabolism, apoptosis, and cell signaling. Defects in lysosomal enzyme activity have been associated with a variety of diseases including Parkinson's, Tay-Sachs, Sandhoff, Krabbe, Wolman, and Gaucher syndromes. These targeted substrates are based upon fluorescent probes that have a low pKa value for optimum fluorescence at the lower physiological pH values found in the lysosomes as well as targeting groups to direct their accumulation to the lysosomes using a live-cell staining format.
This kit is sold by Marker Gene under product code M1376.
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Notes
Important Licensing Information: This product may be covered by one or more United States or International Patents and is subject to our Limited Use Label Licenses. By use of this product, you accept the terms and conditions of all applicable Limited Use Label Licenses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 units LysoLive™ PhosGreen 10 x 50µl Lysosomal Staining Standard 1 x 100µl -
Research areas
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Relevance
Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease. -
Cellular localization
ACP1: Cytoplasm. ACP2: Lysosome membrane; Single-pass membrane protein. ACP5: Lysosome. ACPP: Isoform 1: Secreted. Isoform 2: Lysosome membrane; Single-pass type I membrane protein. -
Alternative names
- Acid phosphatase 1 soluble
- Acid phosphatase 2 lysosomal
- Acid phosphatase 5 tartrate resistant
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