Anti-MTHFD1L antibody (ab116615)
Key features and details
- Goat polyclonal to MTHFD1L
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MTHFD1L antibody
See all MTHFD1L primary antibodies -
Description
Goat polyclonal to MTHFD1L -
Host species
Goat -
Specificity
Based on homology with the immunogen sequence, we believe this antibody will also react with MTHFD1 -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human MTHFD1L aa 550-650 (internal sequence) (Cysteine residue).
Database link: NP_056255.2 -
Positive control
- WB: Recombinant Human MTHFD1 protein (ab116852), human cerebellum tissue lysate, rat and mouse brain tissue lysates and NIH3T3 cell lysate. IHC-P: Human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab116615 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-MTHFD1L antibody (ab116615)Anti-MTHFD1L antibody (ab116615) at 0.1 µg/ml + Human cerebellum tissue lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 106 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour.
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Western blot - Anti-MTHFD1L antibody (ab116615)All lanes : Anti-MTHFD1L antibody (ab116615) at 0.1 µg/ml
Lane 1 : NIH3T3 cell lysate in RIPA buffer
Lane 2 : Mouse brain tissue lysate in RIPA buffer
Lane 3 : Rat brain tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 106 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected by chemiluminescence.
