Recombinant human PD1 protein (Tagged) (Biotin) (ab271670)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, DDDDK tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human PD1 protein (Tagged) (Biotin)
See all PD1 proteins and peptides -
Biological activity
Assay Conditions: Briefly, 50 µl of 2 µg/ml PD-L1 was coated onto plates. 20 µl of 0.5 µg/ml PD-1, FLAG-His, Biotin-labelled was allowed to bind at room temperature for 2 hours. Strep-HRP was added and allowed to incubate at room temp for 1 hr, followed by HPR chemiluminescent substrate addition and plate reading.
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Purity
>= 80 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LDSPDRPWNP PTFSPALLVV TEGDNATFTC SFSNTSESFV LNWYRMSPSN QTDKLAAFPE DRSQPGQDCR FRVTQLPNGR DFHMSVVRAR RNDSGTYLCG AISLAPKAQI KESLRAELRV TERRAEVPTA HPSPSPRPAG QFQ -
Predicted molecular weight
20 kDa including tags -
Molecular weight information
This protein runs at a higher molecular weight by SDS-PAGE due to glycosylation. -
Amino acids
25 to 167 -
Modifications
biotinylated -
Tags
His tag C-Terminus , DDDDK tag C-Terminus , Avi tag C-Terminus
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Conjugation
Biotin
Preparation and Storage
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Alternative names
- CD279
- CD279 antigen
- hPD 1
see all -
Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human PD1 protein (Tagged) (Biotin) (ab271670)SDS-PAGE analysis of ab271670.
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Functional Studies - Recombinant human PD1 protein (Tagged) (Biotin) (ab271670)Binding activity of ab271670.