Recombinant rabbit TIGIT protein (Fc Chimera Active) (ab224645)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Preparation and Storage
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Alternative names
- DKFZp667A205
- ENSMUSG00000071552
- FLJ39873
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Function
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. -
Tissue specificity
Expressed at low levels on peripheral memory and regulatory CD4+ T cells and NK cells and is up-regulated following activation of these cells (at protein level). -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Cellular localization
Cell membrane. - Information by UniProt
Images
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Functional Studies - Recombinant rabbit TIGIT protein (Fc Chimera Active) (ab224645)
Immobilized human Nectin-2, His Tag at 5 μg/ml (100 μl/well) can bind ab224645 with a linear range of 5-78 ng/ml.
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Functional Studies - Recombinant rabbit TIGIT protein (Fc Chimera Active) (ab224645)Loaded ab224645 on Protein A Biosensor, can bind Human Nectin-2, His Tag with an affinity constant of 0.21 μM as determined in BLI assay.
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SDS-PAGE - Recombinant rabbit TIGIT protein (Fc Chimera Active) (ab224645)ab224645 analyzed by SDS-PAGE and stained overnight with Coomassie Blue.
Lane 1: Reducing conditions.
Lane 2: Non-reducing conditions.
As a result of glycosylation, the protein migrates as 45-50 kDa under reducing conditions, and 90-116 kDa under non-reducing conditions.
