Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17685] to BOB1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-BOB1 antibody [EPR17685] - BSA and Azide free
    See all BOB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17685] to BOB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251392 is the carrier-free version of ab203110. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251392 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    Anti-BOB1 antibody [EPR17685] (ab203110) at 1/10000 dilution + RAMOS (Human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 27 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab203110, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The MW profile observed is consistent with what has been described in the literature (PMID: 12857960).

  • Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    Anti-BOB1 antibody [EPR17685] (ab203110) at 1/5000 dilution + Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 27 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab203110, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The MW profile observed is consistent with what has been described in the literature (PMID: 12857960).

  • Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    Western blot - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    All lanes : Anti-BOB1 antibody [EPR17685] (ab203110) at 1/200 dilution

    Lane 1 : Human lymph node whole cell lysate
    Lane 2 : Human bone marrow whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/50000 dilution

    Predicted band size: 27 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab203110, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The MW profile observed is consistent with what has been described in the literature (PMID: 12857960).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    This data was developed using ab203110, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human Non-Hodgkin lymphoma of B- lineage tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weak cytoplasmic staining on Human non-hodgkin lymphoma of B- lineage tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP). Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    This data was developed using ab203110, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Nuclear and weakly cytoplasmic staining on lymphocyte of Human colon lineage tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP). Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    This data was developed using ab203110, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling BOB1 Protein with ab230110 at 1/500 dilution followed by ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody at a 1/500 dilution. Counter stained with Hematoxylin. No staining on Human cerebral cortex tissue (BOB1 Negative tissue) is observed. Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 Goat Anti-Rabbit IgG H&L (HRP). Reference: Greiner A et al. Up-Regulation of BOB.1/OBF.1 Expression in Normal Germinal Center B Cells and Germinal Center-Derived Lymphomas. Am J Pathol. 2000 February; 156(2): 501–507. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)
    Anti-BOB1 antibody [EPR17685] - BSA and Azide free (ab251392)

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