Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CD11a antibody [EPR22578-312] (ab228964)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22578-312] to CD11a
Suitable for: WB, IHC-P
Reacts with: Mouse

Product name

Anti-CD11a antibody [EPR22578-312]
See all CD11a primary antibodies
Description

Rabbit monoclonal [EPR22578-312] to CD11a
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Mouse

WB
Mouse

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: P388D1 and RAW264.7 whole cell lysate. Mouse spleen and thymus lysates. IHC-P: Mouse colon, small intestine and spleen tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Mouse
WB	Mouse

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22578-312
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EPR22578-312] (ab228964)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CD11a with ab228964 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on the immune cells in the mouse colon (PMID: 12434422). The section was incubated with ab228964 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Western blot - Anti-CD11a antibody [EPR22578-312] (ab228964)

Anti-CD11a antibody [EPR22578-312] (ab228964) at 1/1000 dilution + P388D1 (mouse lymphoma monocyte; macrophage), whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 128 kDa
Observed band size: 180 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 2307933) .

Exposure time: 10 seconds.
Western blot - Anti-CD11a antibody [EPR22578-312] (ab228964)

Anti-CD11a antibody [EPR22578-312] (ab228964) at 1/1000 dilution + RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 128 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 2307933) .

Exposure time: 3 minutes.
Western blot - Anti-CD11a antibody [EPR22578-312] (ab228964)

All lanes : Anti-CD11a antibody [EPR22578-312] (ab228964) at 1/1000 dilution

Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse thymus tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 128 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: Lane 1: 125 seconds Lane 2: 7.75 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EPR22578-312] (ab228964)

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling CD11a with ab228964 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on the immune cells in the mouse small intestine. The section was incubated with ab228964 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11a antibody [EPR22578-312] (ab228964)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD11a with ab228964 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on the mouse spleen (PMID: 2185332). The section was incubated with ab228964 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Anti-CD11a antibody [EPR22578-312] (ab228964)

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