Anti-RPA32/RPA2 antibody [9H8] (ab2175)
![Anti-RPA32/RPA2 antibody [9H8] (ab2175)](https://www.abcam.com/ps/products/2/ab2175/Images/ab2175-5440-anti-rpa32-rpa2-antibody-9h8-immunohistochemistry.jpg "Anti-RPA32/RPA2 antibody [9H8] (ab2175)")
Key features and details
- Mouse monoclonal [9H8] to RPA32/RPA2
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RPA32/RPA2 antibody [9H8]
See all RPA32/RPA2 primary antibodies -
Description
Mouse monoclonal [9H8] to RPA32/RPA2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Full length protein (Human).
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Positive control
- WB: TE 671, SK N BE and HeLa whole cell lysates. IHC-P: Human tonsil tissue. Flow Cyt: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
9H8 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining RPA32/RPA2 with ab2175. Staining was detected using DAB.
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![Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175)](https://www.abcam.com/ps/products/2/ab2175/Images/ab2175-5442-anti-rpa32-rpa2-antibody-9h8-western-blot.jpg)
Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175)All lanes : Anti-RPA32/RPA2 antibody [9H8] (ab2175) at 5 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
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![Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)](https://www.abcam.com/ps/products/2/ab2175/Images/ab2175-5441-anti-rpa32-rpa2-antibody-9h8-flow-cytometry.jpg)
Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175) This image is courtesy of an anonymous AbreviewAnti-RPA/32/RPA2 antibody [9H8] (ab2175) is used to measure DNA Double-Stranded Breaks resection in U2OS cells +/- Camptotechin (CPT).
ab2175 is used at 1/200 dilution in 1xPBS + 0.2% Triton X-100 for 1 hour at 25°C.
Secondary antibody: anti-mouse Molecular Probes Alexa Fluor® 488 Conjugated at 1/200 dilution.
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![Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)](https://www.abcam.com/ps/products/2/ab2175/Images/ab2175-5439-anti-rpa32-rpa2-antibody-9h8-flow-cytometry.jpg)
Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)Overlay histogram showing HeLa cells stained with ab2175 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2175, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
