Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TIMP1 antibody [EPR1550] (ab109125)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1550] to TIMP1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human, Recombinant fragment

Product name

Anti-TIMP1 antibody [EPR1550]
See all TIMP1 primary antibodies
Description

Rabbit monoclonal [EPR1550] to TIMP1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

Recombinant fragment

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa cell lysate; HT1080 + TPA, HL60+TPA, PBMC lysates, human prostate lysate; Human liver tissue; TIMP1 recombinant protein
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human Recombinant fragment

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR1550
Isotype

IgG
Research areas

Western blot - Anti-TIMP1 antibody [EPR1550] (ab109125)

All lanes : Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TIMP1 knockout HeLa cell lysate
Lane 3 : HT-1080 treated with 200ng/ml 12-O-Tetradecanoylphorbol-13-acetate (TPA) for 24 hours, cell lysate
Lane 4 : Untreated HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 23 kDa
Observed band size: 26 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab109125 observed at 26 kDa. Red - loading control ab7291 observed at 50 kDa.

ab109125 Anti-TIMP1 antibody [EPR1550] was shown to specifically react with TIMP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261740 (knockout cell lysate ab257291) was used. Wild-type and TIMP1 knockout samples were subjected to SDS-PAGE. ab109125 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR1550] (ab109125)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling TIMP1 with purified ab109125 at 1:8000 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA buffer, pH 9.0. Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR1550] (ab109125)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TIMP1 with purified ab109125 at 1:8000 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA buffer, pH 9.0. Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR1550] (ab109125)

Unpurified ab109125, at 1/250 dilution staining TIMP1 in paraffin-embedded human liver tissue, by Immunohistochemistry.
Western blot - Anti-TIMP1 antibody [EPR1550] (ab109125)

All lanes : Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution (purified)

Lane 1 : Human prostate lysates at 20 µg
Lane 2 : HT-1080 (Human fibrosarcoma epithelial cell) treated with 200ng/ml TPA for 24 hours whole cell lysates at 15 µg
Lane 3 : Untreated with HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates at 15 µg

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 23 kDa

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-TIMP1 antibody [EPR1550] (ab109125)

All lanes : Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution (unpurified)

Lane 1 : HL60+TPA cell lysates
Lane 2 : PBMC cell lysates

Lysates/proteins at 10 µg per lane.

Predicted band size: 23 kDa
Western blot - Anti-TIMP1 antibody [EPR1550] (ab109125)

Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution (unpurified) + TIMP1 recombinant protein at 10 µg

Predicted band size: 23 kDa
Anti-TIMP1 antibody [EPR1550] (ab109125)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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