Anti-Bcl-2 antibody (ab59348)
Key features and details
- Rabbit polyclonal to Bcl-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Bcl-2 antibody
See all Bcl-2 primary antibodies -
Description
Rabbit polyclonal to Bcl-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Bcl-2.
Sequence:The antiserum was produced against synthesized non-phosphopeptide derived from human BCL-2 around the phosphorylation site of threonine 69 (A-R-TP-S-P).
Database link: P10415 -
General notes
Store at -20°C for year
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography, using epitope specific immunogen -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Bcl-2 antibody (ab59348)All lanes : Anti-Bcl-2 antibody (ab59348) at 1/500 dilution
Lane 1 : Extracts from K562 cells with no immunizing peptide
Lane 2 : Extracts from K562 cells with immunizing peptide
Lysates/proteins at 40 µg per lane.
Observed band size: 26 kDa why is the actual band size different from the predicted?Blocking buffer: 5% (w/v) non-fat dry milk in TBST.
Primary antibody dilution buffer: 5%(w/v)non-fat dried milk,0.1%(v/v), Tween-20 in TBST.
Secondary antibody dilution buffer: 5%(w/v)non-fat dried milk,0.1%(v/v),Tween-20 in TBST.
12% SDS gel. Nitrocellulose membrane.
Blocking: Room temperature for 2 hours or overnight at 4°C. Then wash 3x for 5 minutes with 0.05% blocking buffer.
Primary antibody incubation: diluted in TBST at 1/500. Incubate overnight with 4 degrees shaking. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
Secondary antibody incubation: diluted in TBST at 1/2000. Incubate 37°C for 1 hour. Then, in 0.05% TBST, wash membrane 3-4 times for 10min.
ECL development.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody (ab59348)ab59348, at a 1/50 dilution, staining human Bcl-2 in colon carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence (left image) and presence of the immunizing peptide (right image).
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Immunocytochemistry/ Immunofluorescence - Anti-Bcl-2 antibody (ab59348)Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Bcl-2 with ab59348 in the absence (left image) and presence of the immunizing peptide (right image).
