Recombinant Human GLP1R protein (ab130280)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% Densitometry
- Endotoxin level:
- Tags: His tag N-Terminus
- Suitable for: WB, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituent: 0.41% Sodium acetate
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ReconstitutionAdd 0.1M Acetate buffer pH=4.0 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely at 37°C. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10µg/mL.
Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Images
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SDS-PAGE - Recombinant Human GLP1R protein (ab130280)14% SDS-PAGE separation of ab130280:
Lane 1: M.W. marker – 97, 66, 45, 31, 21, 14 kDa
Lane 2: reduced and boiled sample, 5μg/lane.
Lane 3: non-reduced and non-boiled sample, 5μg/lane.
