Anti-Villin antibody (ab272876)
Key features and details
- Rabbit polyclonal to Villin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Villin antibody
See all Villin primary antibodies -
Description
Rabbit polyclonal to Villin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Villin (N terminal). The exact sequence is proprietary.
Database link: P09327 -
Positive control
- WB: Mouse kidney tissue extract, H1299 whole cell lysate. IHC-P: NCIN87 xenograft tissue. ICC/IF: Hep3B cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Villin antibody (ab272876)Anti-Villin antibody (ab272876) at 1/1000 dilution + Mouse kidney tissue at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Villin antibody (ab272876)Paraffin-embedded NCIN87 xenograft tissue stained for Villin using ab272876 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using citrate buffer, pH 6, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-Villin antibody (ab272876)Methanol-fixed Hep3B (human hepatocellular carcinoma cell line) cells stained for Villin using ab272876 at 1/500 dilution in ICC/IF (green).
Co-stained with Hoechst 33342 (blue).
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Western blot - Anti-Villin antibody (ab272876)Anti-Villin antibody (ab272876) at 1/1000 dilution + H1299 (human non-small cell lung carcinoma cell line) whole cell lysate at 30 µg7.5% SDS PAGE