Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)
![Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)](https://www.abcam.com/ps/products/76/ab76155/Images/ab76155-259097-ab76155KOWB.jpg "Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1898-7Y] to Phospholipase C gamma 1/PLC-gamma-1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y]
See all Phospholipase C gamma 1/PLC-gamma-1 primary antibodies -
Description
Rabbit monoclonal [EP1898-7Y] to Phospholipase C gamma 1/PLC-gamma-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide within Human Phospholipase C gamma 1/PLC-gamma-1. The exact sequence is proprietary.
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Positive control
- Jurkat whole cell lysate (ab7899).
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General notes
This product was previously labelled as Phospholipase C gamma 1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1898-7Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Phospholipase C gamma 1/PLC-gamma-1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Mouse brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab76155 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab76155 was shown to specifically react with Phospholipase C gamma 1/PLC-gamma-1 when Phospholipase C gamma 1/PLC-gamma-1 knockout samples were used. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab76155 at a dilution of 1/5000 and ab8245 (loading control to GAPDH) at a dilution of 1/10,000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)](https://www.abcam.com/ps/products/76/ab76155/Images/ab76155wb.gif)
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155) at 1/10000 dilution + Jurkat cell lysates at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDa
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![Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)](https://www.abcam.com/ps/products/76/ab76155/Images/ab76155-4-benefits-of-recombinant-antibodies.png)
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EP1898-7Y] (ab76155)
