Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PD-L1 antibody [EPR20529] (ab213480)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20529] to PD-L1
  • Suitable for: ICC/IF, IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-PD-L1 antibody [EPR20529]
    See all PD-L1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20529] to PD-L1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse placenta lysate; whole cell lysate from RAW 264.7 cells treated with 100ng/ml Interferon-gamma for 48 hours. IP: Mouse placenta lysate. ICC/IF: RAW 264.7 cells treated with IFN-? (100 ng/ml) for 24 hours.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)
    Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)
    All lanes : Anti-PD-L1 antibody [EPR20529] (ab213480) at 1/1000 dilution

    Lane 1 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : Whole cell lysate from RAW 264.7 treated with 100ng/ml Interferon-gamma for 48 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 33 kDa
    Observed band size: 40-60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    IFN-γ treatment induces PD-L1 expression. The molecular weight observed is consistent with what has been described in the literature. PMID: 15353579.

  • Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] (ab213480)
    Immunocytochemistry/ Immunofluorescence - Anti-PD-L1 antibody [EPR20529] (ab213480)

    Ab213480 staining PD-L1 in RAW264.7 from Mouse macrophage cell line transformed with Abelson murine leukemia virus by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% Methanol. Samples were incubated with ab213480 at 0.4 μg/ml. AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as the secondary antibody at 2 μg/ml. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)(ab196889) was used as counterstain at 2.5 μg/ml. DAPI used as nuclear counterstain. Confocal image showing membranous staining on RAW 264.7 cells treated with IFN-γ (100 ng/ml) for 24 hours.

  • Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (ab213480)
    Immunoprecipitation - Anti-PD-L1 antibody [EPR20529] (ab213480)

    PD-L1 was immunoprecipitated from 0.35 mg of Mouse placenta lysate with ab213480 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab213480 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution.

    Lane 1: Mouse placenta lysate, 10 µg (Input).

    Lane 2: ab213480 IP in mouse placenta lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213480 in mouse placenta lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)
    Western blot - Anti-PD-L1 antibody [EPR20529] (ab213480)
    Anti-PD-L1 antibody [EPR20529] (ab213480) at 1/1000 dilution + Mouse placenta lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa
    Observed band size: 40-60 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature. PMID: 15353579.

  • Anti-PD-L1 antibody [EPR20529] (ab213480)
    Anti-PD-L1 antibody [EPR20529] (ab213480)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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