Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ETFDH antibody [3D1AC4AF3] (ab131376)

Key features and details

  • Mouse monoclonal [3D1AC4AF3] to ETFDH
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-ETFDH antibody [3D1AC4AF3]
    See all ETFDH primary antibodies

  • Description

    Mouse monoclonal [3D1AC4AF3] to ETFDH

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: HLH, HHH, HeLa, HDFn, SY5Y, Jurkat, MLH, MHH, RLH, RHH, RBH, RKH, RSMH tissue homogenates
      ICC/IF: Paraformaldehyde fixed HDFn (Human), NIH 3T3 (Mouse) and H9C2 (Rat) cells
      Flow Cyt: HeLa cells This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
    Western blot - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
    All lanes : Anti-ETFDH antibody [3D1AC4AF3] (ab131376) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Human Liver Homogenate (HLH) at 10 µg
    Lane 3 : Human Heart Homogenate (HHH) at 10 µg
    Lane 4 : HepG2 Cell Lysate at 15 µg
    Lane 5 : Hela Cell Lysate at 15 µg
    Lane 6 : HDFn Cell Lysate at 15 µg
    Lane 7 : SY5Y Cell Lysate at 15 µg
    Lane 8 : Jurkat Cell Lysate at 15 µg
    Lane 9 : Mouse Liver Homogenate (MLH) at 10 µg
    Lane 10 : Mouse Heart Homogenate (MHH) at 10 µg
    Lane 11 : Rat Liver Homogenate (RLH) at 10 µg
    Lane 12 : Rat Heart Homogenate (RHH) at 10 µg
    Lane 13 : Rat Brain Homogenate (RBH) at 10 µg
    Lane 14 : Rat Kidney Homogenate (RLH) at 10 µg
    Lane 15 : Rat Skeletal Muscle Homogenate (RSMH) at 10 µg

    Secondary
    All lanes : Goat anti-Mouse AP at 1/3000 dilution

    Predicted band size: 68 kDa



    The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the Goat anti-Mouse secondary antibody.
  • Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
    Immunocytochemistry/ Immunofluorescence - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
    Immunofluorescent analysis of ETFDH in HDFn cells.
    The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were then incubated with ab131376 at 5 µg/ml for 2 h at room temperature, or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1 h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the mitochondria.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)

    IHC image of ETFDH staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131376, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
    Flow Cytometry - Anti-ETFDH antibody [3D1AC4AF3] (ab131376)
    Flow cytometric analysis of ETFDH in HeLa cells fixed and permeabilized with methanol and stained with 1 µg/mL of ab131376 (blue), or an equal amount of an isotype control antibody (red). 1% BSA was used as the blocking reagent for all the blocking steps.

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