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Anti-CD44 antibody [C44Mab-5] (ab264539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [C44Mab-5] to CD44
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD44 antibody [C44Mab-5]
    See all CD44 primary antibodies

  • Description

    Mouse monoclonal [C44Mab-5] to CD44

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • WB: MDA-MB-231 whole cell lysate. IHC-P: Human lung carcinoma and skin tissue. Flow: MDA-MB-231 cells

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-CD44 antibody [C44Mab-5] (ab264539)
    Western blot - Anti-CD44 antibody [C44Mab-5] (ab264539)
    All lanes : Anti-CD44 antibody [C44Mab-5] (ab264539) at 1.226 µg/ml

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 81 kDa
    Observed band size: 82 kDa
    why is the actual band size different from the predicted?


    Exposure time: 70 seconds


    Blocking/diluting buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264539).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] (ab264539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] (ab264539)

    Formalin-fixed, paraffin-embedded Human lung carcinoma tissue stained for CD44 using ab264539 at 0.253 µg/mL followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) in immunohistochemical analysis. Counterstained with Hematoxylin. Membranous staining on Human lung carcinoma. The section was incubated with ab264539 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    Secondary antibody only control: Used PBS instead of the primary antibody, secondary antibody was a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] (ab264539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [C44Mab-5] (ab264539)

    Formalin-fixed, paraffin-embedded Human skin tissue stained for CD44 using ab264539 at 0.253 µg/mL followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) in immunohistochemical analysis. Counterstained with Hematoxylin. Membranous staining on human skin. The section was incubated with ab264539 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. 

    Secondary antibody only control: Used PBS instead of the primary antibody, secondary antibody was a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  • Flow Cytometry - Anti-CD44 antibody [C44Mab-5] (ab264539)
    Flow Cytometry - Anti-CD44 antibody [C44Mab-5] (ab264539)

    Flow cytometric analysis of MDA-MB-231 (Human breast adenocarcinoma epithelial cell) cell line labeling CD44 (Red) using ab264539 at 1.055 µg/mL followed by Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution. Mouse monoclonal IgG was used as the isotype control (Black). Cells without incubation with primary antibody and secondary antibody (Blue).

    Gated on viable cells.

     

  • Anti-CD44 antibody [C44Mab-5] (ab264539)
    Anti-CD44 antibody [C44Mab-5] (ab264539)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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