Anti-p53 (mono methyl K372) antibody (ab227470)
Key features and details
- Rabbit polyclonal to p53 (mono methyl K372)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-p53 (mono methyl K372) antibody
See all p53 primary antibodies -
Description
Rabbit polyclonal to p53 (mono methyl K372) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Rhesus monkey -
Immunogen
Synthetic peptide within Human p53 (C terminal) (mono methyl K372). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: P04637 -
Positive control
- IHC-P: Human cervical cancer tissue. ICC/IF: U-2 OS cells. WB: HCT 116, treated with 30uM cisplatin for 24hours, whole cell lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-p53 (mono methyl K372) antibody (ab227470) at 1/500 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) cells mock treated, whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) cells treated with 30 uM cisplatin for 24 hour, whole cell lysate
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 44 kDa10% SDS-PAGE gel.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p53 (mono methyl K372) antibody (ab227470)
Paraffin-embedded human cervical cancer tissue stained for p53 (mono methyl K372) using ab227470 at 1/500 dilution in immunohistochemical analysis.
-
Paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for p53 (mono methyl K372) (green) using ab227470 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an anti-alpha-tubulin antibody at 1/2000 dilution (red).