Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP6V1A antibody [EPR19271] (ab199325)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19271] to ATP6V1A
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATP6V1A antibody [EPR19271]
    See all ATP6V1A primary antibodies

  • Description

    Rabbit monoclonal [EPR19271] to ATP6V1A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, fetal heart and fetal kidney lysates; HeLa, K562, HEK-293, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates; Mouse brain, kidney and spleen lysates; Rat brain, kidney and spleen lysates. IHC-P: Human kidney and breast cancer tissues; Mouse cerebrum and rat kidney tissues. IP: HeLa whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19271

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Western blot - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    All lanes : Anti-ATP6V1A antibody [EPR19271] (ab199325) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 8 seconds; Lane 3: 3 seconds

  • Western blot - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Western blot - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    All lanes : Anti-ATP6V1A antibody [EPR19271] (ab199325) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Western blot - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    All lanes : Anti-ATP6V1A antibody [EPR19271] (ab199325) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 1 second; Lane 3: 3 seconds; Lane 4 and 5: 1 second; Lane 6: 3 seconds.

     

  • Western blot - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Western blot - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    All lanes : Anti-ATP6V1A antibody [EPR19271] (ab199325) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa
    Observed band size: 68 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2 and 3: 3 seconds; Lane 4: 1 second.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1A antibody [EPR19271] (ab199325)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ATP6V1A with ab199325 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kidney tubules of normal Human kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1A antibody [EPR19271] (ab199325)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling ATP6V1A with ab199325 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on cancer cells of the breast is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1A antibody [EPR19271] (ab199325)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ATP6V1A with ab199325 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on neurons of the mouse cerebrum is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1A antibody [EPR19271] (ab199325)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ATP6V1A with ab199325 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on kidney tubules of the rat kidney is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Immunoprecipitation - Anti-ATP6V1A antibody [EPR19271] (ab199325)

    ATP6V1A was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab199325 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab199325 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: HeLa whole cell lysate 10µg (Input).
    Lane 2: ab199325 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199325 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

  • Anti-ATP6V1A antibody [EPR19271] (ab199325)
    Anti-ATP6V1A antibody [EPR19271] (ab199325)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-ATP6V1A antibody [EPR19271] (ab199325)

© 2021 Astana Biomed Group LLP. All rights reserved.